One Step Multiplex PCR for Identifications at Subspecies Level of <i>Fusobacterium nucleatum</i> and <i>Fusobacterium necrophorum</i>

Multiplex PCR: critical parameters and step-by-step protocol.

By simultaneously amplifying more than one locus in the same reaction, multiplex PCR is becoming a rapid and convenient screening assay in both the clinical and the research laboratory. While numerous papers and manuals discuss in detail conditions influencing the quality of PCR in general, relatively little has been published about the important experimental factors and the common difficulties...

Designing and Validation of One-Step T-ARMS-PCR for Genotyping the eNOS rs1799983 SNP

Background: The transversion of G to T (G894T) in human endothelial nitric oxide synthase (eNOS) gene has profound effects such as male infertility, recurrent miscarriage, multiple sclerosis and cardiovascular diseases.Objectives: Development of a new Multiplex Tetra-Primer Amplifi cation Refractory Mutation System - Polymerase Chain Reaction (T-ARMS-PCR) for detection of...

A multiplex one-step real-time RT-PCR assay for influenza surveillance.

For surveillance purposes real-time PCR assays for influenza viruses had to be adapted to the pandemic influenza A(H1N1)2009 strain. We combined published primers and probes for influenza A, influenza B and an internal amplification control with a detection system for influenza A(H1N1)2009 to set up a rapid, reliable, simple and cost-effective high-throughput multiplex one-step real-time RT-PCR...

One-step multiplex reverse-transcriptase PCR for detecting pandemic (H1N1) 2009 influenza virus.

Pandemic (H1N1) 2009 influenza has spread throughout the world since April 2009 and has caused many human deaths since its first report in humans. Pandemic (H1N1) 2009 influenza virus was first identified in a Canadian pig herd in April 2009 and has been reported in more than ten countries, including Korea. We developed a one-step multiplex reverse transcriptase polymerase chain reaction (RT-PC...

One-Step Reverse Transcription PCR (RT-PCR). Fatemeh Mehrpooyan*